Clinical Utility of Early Viral Load Quantification

Clinical utility of viral load in management of cytomegalovirus infection after solid organ transplantation.

The negative impact of cytomegalovirus (CMV) infection on transplant outcomes warrants efforts toward improving its prevention, diagnosis, and treatment. During the last 2 decades, significant breakthroughs in diagnostic virology have facilitated remarkable improvements in CMV disease management. During this period, CMV nucleic acid amplification testing (NAT) evolved to become one of the most ...

The clinical utility of viral load monitoring in HIV infection: strengths and limitations.

Quantification of HIV-1 viral load in the fluid of ranulas in HIV-positive patients.

OBJECTIVE This study aimed to detect and quantify the HIV-1 viral load in ranula fluid of HIV-positive patients. STUDY DESIGN Fourteen HIV-positive patients (13 not on highly active antiretroviral therapy) presenting with ranulas were prospectively evaluated. The viral load in the ranula fluid was quantified, and the results were correlated with CD4(+) cell count and viral load in the patient...

Performance of a Taqman Assay for Improved Detection and Quantification of Human Rhinovirus Viral Load

Human rhinovirus (HRV) is the major aetiology of respiratory tract infections. HRV viral load assays are available but limitations that affect accurate quantification exist. We developed a one-step Taqman assay using oligonucleotides designed based on a comprehensive list of global HRV sequences. The new oligonucleotides targeting the 5'-UTR region showed high PCR efficiency (E = 99.6%, R2 = 0....

Essentials of filoviral load quantification.

Quantitative measurement of viral load is an important parameter in the management of filovirus disease outbreaks because viral load correlates with severity of disease, survival, and infectivity. During the ongoing Ebola virus disease outbreak in parts of Western Africa, most assays used in the detection of Ebola virus disease by more than 44 diagnostic laboratories yielded qualitative results...